Difference between revisions of "Part:BBa K3505014"

(→‎Usage and Biology)
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Constitutive Anderson <bbpart>BBa_J23115</bbpart>with <i>Tet Operator</i>  and RBS <bbpart>BBa_B0034</bbpart>
 
Constitutive Anderson <bbpart>BBa_J23115</bbpart>with <i>Tet Operator</i>  and RBS <bbpart>BBa_B0034</bbpart>
  
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[[File:T--Thessaly--anderson.png|700px|thumb|none|<i><b>Fig.1:</b>J23115 with TetO and RBS</i>]]
 
  
  
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The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning.  
 
The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning.  
 
This has position A1-B3.
 
This has position A1-B3.
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[[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the Golden Braid Grammar</i>]]
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[[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.1:</b>The overhangs of this part in the Golden Braid Grammar</i>]]
  
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===Verification of cloning===
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[[Image:T--Thessaly--andersontetO-snap.png|900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- AndersonJ23115:TetO:RBS (illustration from SnapGene)</i>]]
  
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===Verification of cloning===
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[[File:T--Thessaly--TET0-digestion.png |700px|thumb|none|<i><b>Fig.1:</b>Figure 7: (U=Uncut , C= Cut) Restriction digestion of TetO (C1-C4 ) with :Not-HF(C1-C4) , Expected bands : 2046 + 129 bp, Positive result: C1,C2,C4</i>]]
  
 
===Experimental Use and Experience===
 
===Experimental Use and Experience===

Revision as of 11:21, 26 October 2020


AndersonJ23115:TetO:RBS GB compatible with A1-B2

Constitutive Anderson BBa_J23115with Tet Operator and RBS BBa_B0034



Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. This has position A1-B3.

Fig.1:The overhangs of this part in the Golden Braid Grammar
Figure 2. The level 0 module : pupd2- AndersonJ23115:TetO:RBS (illustration from SnapGene)

Verification of cloning

Fig.1:Figure 7: (U=Uncut , C= Cut) Restriction digestion of TetO (C1-C4 ) with :Not-HF(C1-C4) , Expected bands : 2046 + 129 bp, Positive result: C1,C2,C4

Experimental Use and Experience

This part is used in BBa_K3505034

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]