Difference between revisions of "Part:BBa K3505013"
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[[File:T--Thessaly--anderson.png|700px|thumb|none|<i><b>Fig.1:</b>J23115 with LacO and RBS</i>]] | [[File:T--Thessaly--anderson.png|700px|thumb|none|<i><b>Fig.1:</b>J23115 with LacO and RBS</i>]] | ||
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The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning. | The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning. | ||
This has position A1-B3. | This has position A1-B3. | ||
| − | + | [[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the Golden Braid Grammar</i>]] | |
===Verification of cloning=== | ===Verification of cloning=== | ||
Revision as of 10:24, 26 October 2020
AndersonJ23115:LacO:RBS GB compatible A1-B2
Constitutive Anderson BBa_J23115with Lac Operator and RBS BBa_B0034
File:T--Thessaly--anderson.png
Fig.1:J23115 with LacO and RBS
Usage and Biology
Design Notes
The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. This has position A1-B3.
Verification of cloning
Experimental Use and Experience
This part is used in BBa_K3505032
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]