Difference between revisions of "Part:BBa K3505014"

 
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<partinfo>BBa_K3505014 short</partinfo>
 
<partinfo>BBa_K3505014 short</partinfo>
  
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long
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Constitutive Anderson <bbpart>BBa_J23115</bbpart>with <i>Tet Operator</i>  and RBS <bbpart>BBa_B0034</bbpart>
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[[File:T--Thessaly--anderson.png|700px|thumb|none|<i><b>Fig.1:</b>J23115 with TetO and RBS</i>]]
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K3505014 SequenceAndFeatures</partinfo>
 
  
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===Design Notes===
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The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning.
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This has position A1-B3.
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[[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the Golden Braid Grammar</i>]]
  
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<!-- Uncomment this to enable Functional Parameter display
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===Verification of cloning===
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===Functional Parameters===
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<partinfo>BBa_K3505014 parameters</partinfo>
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===Experimental Use and Experience===
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This part is used in <bbpart>BBa_K3505034</bbpart>
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===Sequence and Features===
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<partinfo>BBa_K3505014 SequenceAndFeatures</partinfo>

Revision as of 10:23, 26 October 2020


AndersonJ23115:TetO:RBS GB compatible with A1-B2

Constitutive Anderson BBa_J23115with Tet Operator and RBS BBa_B0034

File:T--Thessaly--anderson.png
Fig.1:J23115 with TetO and RBS



Usage and Biology

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. This has position A1-B3.

Fig.2:The overhangs of this part in the Golden Braid Grammar

Verification of cloning

Experimental Use and Experience

This part is used in BBa_K3505034

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]