Difference between revisions of "Part:BBa K3425025"
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<p>This sequence should be cloned to </html>[[Part:pSB1K03|<partinfo>pSB1K03</partinfo>]]<html> and used for cloning into pEven plasmids. For the sequence ready to clone with the correct restriction sites, visit Team UofUppsala 2020's <a href="https://2020.igem.org/Team:UofUppsala/Parts">Parts page</a>. For more information about Type IIS standard assembly, you can read Team UofUppsala 2020's <a href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">Type IIS guidebook</a>.</p> | <p>This sequence should be cloned to </html>[[Part:pSB1K03|<partinfo>pSB1K03</partinfo>]]<html> and used for cloning into pEven plasmids. For the sequence ready to clone with the correct restriction sites, visit Team UofUppsala 2020's <a href="https://2020.igem.org/Team:UofUppsala/Parts">Parts page</a>. For more information about Type IIS standard assembly, you can read Team UofUppsala 2020's <a href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">Type IIS guidebook</a>.</p> | ||
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+ | This part is part of iGEM Uppsala 2020's <a href="https://parts.igem.org/Collections/iGEM_Type_IIS_Collections">iGEM Type IIS standard collection</a>. | ||
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Latest revision as of 21:11, 25 October 2020
iGEM Type IIS pSB1K03-DY
pSB1K03-DY is a dummy sequence for iGEM Type IIS standard (RFC1000). This part mimics a transcriptional unit (TU3) and can be included in the assembly whenever necessary.
This sequence should be cloned to pSB1K03 and used for cloning into pEven plasmids. For the sequence ready to clone with the correct restriction sites, visit Team UofUppsala 2020's Parts page. For more information about Type IIS standard assembly, you can read Team UofUppsala 2020's Type IIS guidebook.
This part is part of iGEM Uppsala 2020's iGEM Type IIS standard collection.The following table details the part numbers and names of other pOdd dummies (which contain the plasmid they should be cloned into), along with the transcriptional unit they are mimicking.
Registry Number | Name | Mimics |
---|---|---|
BBa_K3425023 | pSB1K01-DY | TU1 |
BBa_K3425024 | pSB1K02-DY | TU2 |
BBa_K3425025 | pSB1K03-DY | TU3 |
BBa_K3425026 | pSB1K04-DY | TU4 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]