Difference between revisions of "Part:BBa K3457020"

 
Line 3: Line 3:
 
<partinfo>BBa_K3457020 short</partinfo>
 
<partinfo>BBa_K3457020 short</partinfo>
  
−
CAHS 106094 with AAV tag
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;This biological is the CDS sequence of Cytosolic-abundant heat soluble protein (CAHS) 106094 with an AAV tag. AAV tag promotes the degradation of CAHS 106094. </p>
 +
 
 +
===Contribution===
 +
<h2><b>Group: QHFZ-China iGEM 2020</b></h2>
 +
<h3><b>Author: Yixian Yang</b></h3>
 +
<h3><b>Design</b></h3>
 +
<p style="clear:left;">&nbsp;&nbsp;&nbsp;&nbsp;This part is the CDS of CAHS 106094([https://parts.igem.org/Part:BBa_K3457012 BBa_K3457012]) with an AAV tag at the end. This year, the part is used in the composite part [https://parts.igem.org/Part:BBa_K3457045 BBa_K3457045]. </p>
 +
<h3><b>Documentation:</b></h3>
 +
<h4><b>Results:</b></h4>
 +
<p style="clear:left;">&nbsp;&nbsp;&nbsp;&nbsp;The method to induce the expression of CAHS 106094 was the sanme as that in [https://parts.igem.org/Part:BBa_K3457012 BBa_K3457012]. Via SDS-PAGE, we observed the band of CAHS 106094 was obvious, but CAHS 106094-AAV was difficult to perceive (Fig.1). The result implied that AAV tag may promote the degradation of CAHS 106094.</p>
 +
 
 +
[[File:T--QHFZ-China--AAV.png|400px|thumb|left|Figure 1. AAV tag may promote the degradation of CAHS 106094.]]
 +
 
 +
<p style="clear:left;"></p>
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:05, 25 October 2020


CAHS 106094 with a 6X His tag and an AAV tag

    This biological is the CDS sequence of Cytosolic-abundant heat soluble protein (CAHS) 106094 with an AAV tag. AAV tag promotes the degradation of CAHS 106094.

Contribution

Group: QHFZ-China iGEM 2020

Author: Yixian Yang

Design

    This part is the CDS of CAHS 106094(BBa_K3457012) with an AAV tag at the end. This year, the part is used in the composite part BBa_K3457045.

Documentation:

Results:

    The method to induce the expression of CAHS 106094 was the sanme as that in BBa_K3457012. Via SDS-PAGE, we observed the band of CAHS 106094 was obvious, but CAHS 106094-AAV was difficult to perceive (Fig.1). The result implied that AAV tag may promote the degradation of CAHS 106094.

Figure 1. AAV tag may promote the degradation of CAHS 106094.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]