Composite

Part:BBa_K3457045

Designed by: Yixian Yang   Group: iGEM20_QHFZ-China   (2020-10-04)


J23100-CAHS 106094-AAV

    this biological part is the expression sequence of Cytosolic-abundant heat soluble protein (CAHS) 106094 with an AAV tag. AAV tag promotes the degradation of CAHS 106094.

Contribution

Group: QHFZ-China iGEM 2020

Author: Yixian Yang

Design

    This part is the alomost the same as J23100-Olac-RBS-CAHS 106094 (BBa_K3457046) with an AAV tag at the end of the CDS of CAHS 106094.

Documentation:

Results:

    The method to induce the expression of CAHS 106094 was the same as that in BBa_K3457046. Via SDS-PAGE, we observed the band of CAHS 106094 was obvious, but CAHS 106094-AAV was difficult to perceive (Fig.1). The result implied that AAV tag may promote the degradation of CAHS 106094.

Figure 1. AAV tag may promote the degradation of CAHS 106094.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
    Illegal NheI site found at 978
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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