Difference between revisions of "Part:BBa K3505032"
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<partinfo>BBa_K3505032 short</partinfo> | <partinfo>BBa_K3505032 short</partinfo> | ||
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− | < | + | eCFP <bbpart>BBa_K3505020</bbpart>uder control of a constitutive promoter andersonJ23115 having downstream a lac operator <bbpart>BBa_K2924013</bbpart>. |
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+ | [[File:T--Thessaly--lacoecfpmap.png|600px|thumb|none|<i><b>Fig.1:</b>pFliC-LacI-Terminator</i>]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This Trancriscription Unit (TU) is continuesly activated exressing the eCFP protein as a reporter. The Lac operator that is downsteam the anderson exists for the lac regulated inhibition. | ||
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+ | ===Design Notes=== | ||
+ | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in both a1R <bbpart>BBa_K3505008</bbpart>and a2 <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for Golden Braid cloning. | ||
− | + | ===Verification of cloning=== | |
− | === | + | [[File:T--Thessaly--lacoecfp.png|600px|thumb|none|<i><b>Fig.2:</b>(U=Uncut C=Cut) Restriction Enzyme with HindIII. Expected bands in bp 2847 + 804 + 146</i>]] |
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− | < | + | ===Experimental Use and Experinece=== |
+ | This part is used in <bbpart>BBa_K3505036</bbpart> | ||
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+ | ===Extra Engineering Use=== | ||
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+ | ===Sequence and Features=== | ||
+ | <partinfo>BBa_K3505032 SequenceAndFeatures</partinfo> |
Revision as of 10:27, 25 October 2020
pAndersonJ23115:lacO:RBS-eCFP -terminator
eCFP BBa_K3505020uder control of a constitutive promoter andersonJ23115 having downstream a lac operator BBa_K2924013.
Usage and Biology
This Trancriscription Unit (TU) is continuesly activated exressing the eCFP protein as a reporter. The Lac operator that is downsteam the anderson exists for the lac regulated inhibition.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in both a1R BBa_K3505008and a2 BBa_K3505009 and has overhangs compatible for Golden Braid cloning.
Verification of cloning
Experimental Use and Experinece
This part is used in BBa_K3505036
Extra Engineering Use
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]