Difference between revisions of "Part:BBa K103020:Experience"
| Line 8: | Line 8: | ||
This part was used as core of prey generator device. It was fused with protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. Resulting fusion was named His_omega_Z | This part was used as core of prey generator device. It was fused with protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. Resulting fusion was named His_omega_Z | ||
| − | The fusion was overexpressed in | + | The fusion was overexpressed in Rosetta strain. Due to ability of protein Z to form aggregates His_omega_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. |
The fusion was added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]]. | The fusion was added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]]. | ||
Latest revision as of 12:47, 28 October 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K103020
'Hunter and prey' selection system
This part was used as core of prey generator device. It was fused with protein ZSPA-1 BBa_K103004. Resulting fusion was named His_omega_Z
The fusion was overexpressed in Rosetta strain. Due to ability of protein Z to form aggregates His_omega_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. The fusion was added to culture media and used in selection experiments. Details here and here.
User Reviews
UNIQ7383b7e1c994fc8f-partinfo-00000000-QINU UNIQ7383b7e1c994fc8f-partinfo-00000001-QINU