Difference between revisions of "Part:BBa K3505003"
Line 10: Line 10:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'> ===Sequence and Features===</span>
+
<span class='h3bb'> Sequence and Features</span>
 
<partinfo>BBa_K3505003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3505003 SequenceAndFeatures</partinfo>

Revision as of 15:02, 9 October 2020

LacI-Lac repressor GB compatible with B3-B5

Coding region for the LacI protein without an RBS.

Usage and Biology

The lac inhibitor and its operator is usually use for gene regulation and together they form a genetic circuit which called : the lac operon. In bacteria ,in abundance of lactose , lactose binds to lacI and the lac operon is activated. In synthetic biology , as an inducer we use IPTG, which doesn’t degraded in the cell , instead of lactose . IPTG is a molecular mimic of a lactose metabolite, which activates transcription of the lac operon. The lacI has the ability to bind to the lac operator (BBa_R0010 and BBa_R0011 hybrid regulator) inhibiting the expression of the desirable transcription unit.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]