Difference between revisions of "Part:BBa K3610020:Design"
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===Source=== | ===Source=== | ||
| − | The sequence was taken of Part:BBa_K1093016 from the iGEM registry and then codon optimized with SnapGene. | + | The sequence was taken of [[Part:BBa_K1093016]] from the iGEM registry and then codon optimized with SnapGene. |
===References=== | ===References=== | ||
Revision as of 19:34, 4 October 2020
mCherry N-terminal - codon optimized for S. cerevisiae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standard.
Source
The sequence was taken of Part:BBa_K1093016 from the iGEM registry and then codon optimized with SnapGene.