Difference between revisions of "Part:BBa K3610003"

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<partinfo>BBa_K3610003 short</partinfo>
 
<partinfo>BBa_K3610003 short</partinfo>
  
The BAK1 cell surface receptor from A. thaliana [[Part:BBa_K3610004]] consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a protein kinase.
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This part is a version of the BAK1 receptor ([[Part:BBa_K3610001]] for full length) without the intracellular kinase domain and without the native signal sequence.
For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output.
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This part has the is the BAK1 receptor without the kinase domain and without the native signal sequence.
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===Usage and Biology===
 
===Usage and Biology===
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The BAK1 cell surface receptor from A. thaliana consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a kinase domain and initiates a signal cascade upon dimerization with a plant PRR to activate the plant immune response.
 +
 +
 +
For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output.
 +
In our iGEM project, we fused this sequence to a split-mCherry protein and a split-luciferase and then coexpressed the part with another plant PRR carrying the counterpart to the split protein instead of the intracellular kinase domain.
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Revision as of 18:26, 30 September 2020


BAK1 Ectodomain from Arabidopsis thaliana

This part is a version of the BAK1 receptor (Part:BBa_K3610001 for full length) without the intracellular kinase domain and without the native signal sequence.


Usage and Biology

The BAK1 cell surface receptor from A. thaliana consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a kinase domain and initiates a signal cascade upon dimerization with a plant PRR to activate the plant immune response.


For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output. In our iGEM project, we fused this sequence to a split-mCherry protein and a split-luciferase and then coexpressed the part with another plant PRR carrying the counterpart to the split protein instead of the intracellular kinase domain.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]