Difference between revisions of "Part:BBa K143082"
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<partinfo>BBa_K143082 short</partinfo> | <partinfo>BBa_K143082 short</partinfo> | ||
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RFP gene under expresson of the Pveg promoter and spoVG RBS of ''B. subtilis'', with a chloramphenicol resistance marker for ease of selection. | RFP gene under expresson of the Pveg promoter and spoVG RBS of ''B. subtilis'', with a chloramphenicol resistance marker for ease of selection. | ||
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Also contains the 5' and 3' amyE integration sequences to allow integration into ''B. subtilis'' at the amyE locus. | Also contains the 5' and 3' amyE integration sequences to allow integration into ''B. subtilis'' at the amyE locus. | ||
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This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part <bbpart>BBa_K143053</bbpart>) as part of the Biofabricator ''subtilis'' project. | This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part <bbpart>BBa_K143053</bbpart>) as part of the Biofabricator ''subtilis'' project. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><big>'''Sequence and Features'''</big></span> |
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<partinfo>BBa_K143082 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143082 SequenceAndFeatures</partinfo> | ||
Revision as of 18:53, 27 October 2008
Pveg-spoVG RFP expression construct
RFP gene under expresson of the Pveg promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.
Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.
This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part BBa_K143053) as part of the Biofabricator subtilis project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2136
Illegal AgeI site found at 2248 - 1000COMPATIBLE WITH RFC[1000]