Difference between revisions of "Part:BBa K143079"
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<partinfo>BBa_K143079 short</partinfo> | <partinfo>BBa_K143079 short</partinfo> | ||
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GFP gene under expresson of the Pveg promoter and spoVG RBS of ''B. subtilis'', with a chloramphenicol resistance marker for ease of selection. | GFP gene under expresson of the Pveg promoter and spoVG RBS of ''B. subtilis'', with a chloramphenicol resistance marker for ease of selection. | ||
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Also contains the 5' and 3' amyE integration sequences to allow integration into ''B. subtilis'' at the amyE locus. | Also contains the 5' and 3' amyE integration sequences to allow integration into ''B. subtilis'' at the amyE locus. | ||
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This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part <bbpart>BBa_K143053</bbpart>) as part of the Biofabricator ''subtilis'' project. | This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part <bbpart>BBa_K143053</bbpart>) as part of the Biofabricator ''subtilis'' project. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><big>'''Sequence and Features'''</big></span> |
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<partinfo>BBa_K143079 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143079 SequenceAndFeatures</partinfo> | ||
Revision as of 18:45, 27 October 2008
Pveg-spoVG GFP expression construct
GFP gene under expresson of the Pveg promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.
Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.
This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part BBa_K143053) as part of the Biofabricator subtilis project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2225