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==Functional Parameters: Austin_UTexas==
 
==Functional Parameters: Austin_UTexas==
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<partinfo>BBa_I712058 parameters</partinfo>
 
 
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Latest revision as of 00:04, 4 September 2020


CMVp - myri - TEVP - GFP

Signal sequence for myristoilation fused with proteolytic cleavage site for TEV protease and mCerulean, all under control of CMV promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters

abs-NA-
emission-NA-
emit476
excitation-NA-
excite439
lum-NA-
proteinECFP-ASV
tagASV


Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 3.4 ± 0.5%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.