Difference between revisions of "Part:BBa K3110054"
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<h1>Characterization</h1> | <h1>Characterization</h1> | ||
| − | This part was used as a control for O1PO2-StrongRBS-YebF-IL12-FLAG ([[Part:BBa_K3110051]]) to check the | + | This part was used as a control for O1PO2-StrongRBS-YebF-IL12-FLAG ([[Part:BBa_K3110051]]) to check the difference between YebF-IL12 produced by the inducible promoter and constitutive Promoter (without the operator regions). |
The results for this construct can be found with the Result of the sample construct ([[Part:BBa_K3110051]]) | The results for this construct can be found with the Result of the sample construct ([[Part:BBa_K3110051]]) | ||
Latest revision as of 02:55, 22 October 2019
J23117-YebF IL12-FLAG
The original YebF part can be found here: BBa_K1659003
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
YebF is a 13 kDa protein that is known to be secreted into the extracellular medium by E. coli strains used in the lab. This particular protein was conjugated with IL-12 in our design, in order to mediate the secretion of the cytokine from the bacteria. The part was used with a constitutive promoter (J23100) but the results were inconclusive.SDS-PAGE was done with both the cell pellet and concentrated supernatant of our test sample and a control (untransformed BL21) but no visible band difference was detected. We suspected this could be because of SOEing errors in the promoter and RBS regions.
Characterization
This part was used as a control for O1PO2-StrongRBS-YebF-IL12-FLAG (Part:BBa_K3110051) to check the difference between YebF-IL12 produced by the inducible promoter and constitutive Promoter (without the operator regions). The results for this construct can be found with the Result of the sample construct (Part:BBa_K3110051)