Difference between revisions of "Part:BBa K3117048:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | ... | + | By connecting the variable regions of the heavy and the light chain of an anti-CD3 antibody with a short GGGGS linker (<partinfo>BBa_K3117004</partinfo>), the scFv retains it's antigen-binding ability and is much smaller than a conventional antibody. Thus, it is well suited as part of a fusion protein with another effector. The SpyTag attached to the scFv belongs to the SpyTag/SpyCatcher system and is one part of the FbaB protein of Streptococcus pyogenes. Once it comes into contact with its corresponding other part, the SpyCatcher (<partinfo>BBa_K3117016</partinfo>), they bind covalently. This allows our part to be used in a modular manner in combination with other molecules carrying the SpyCatcher. The sequence contains a C-terminal His-tag (<partinfo>BBa_K3117005</partinfo>) for easy purification and detection. Secretion of the protein into the periplasm is ensured by the pelB sequence (<partinfo>BBa_K3117012</partinfo>) |
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===Source=== | ===Source=== | ||
Latest revision as of 22:14, 21 October 2019
scFv against CD3 with SpyTag codon optimized for E.coli
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By connecting the variable regions of the heavy and the light chain of an anti-CD3 antibody with a short GGGGS linker (BBa_K3117004), the scFv retains it's antigen-binding ability and is much smaller than a conventional antibody. Thus, it is well suited as part of a fusion protein with another effector. The SpyTag attached to the scFv belongs to the SpyTag/SpyCatcher system and is one part of the FbaB protein of Streptococcus pyogenes. Once it comes into contact with its corresponding other part, the SpyCatcher (BBa_K3117016), they bind covalently. This allows our part to be used in a modular manner in combination with other molecules carrying the SpyCatcher. The sequence contains a C-terminal His-tag (BBa_K3117005) for easy purification and detection. Secretion of the protein into the periplasm is ensured by the pelB sequence (BBa_K3117012)
Source
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