Difference between revisions of "Part:BBa K3110051:Design"

Latest revision as of 21:20, 21 October 2019

lldRO1-J23117-lldRO2 Strong RBS YebF-IL12

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 78
Illegal NheI site found at 101
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

IL12 and YebF was two different parts used by two different iGEM teams. In order to improve the part used by Brazil (BBa_K554005), we have codon optimized it to make it synthesize compatible and attached a Secretory peptide YebF so that IL12 also get secreted along with YebF

So, the whole part contains:

The Promoter and Operator Region: lldRO1-J23117-lldRO2 (Part:BBa_K1847008)
A strong RBS: Part:BBa_B0034
Gene Construct: YebF-IL12-FLAG (Part:BBa_K3110050)
Double terminator: Part:BBa_B0015

Source

YebF-IL12-FLAG tag was synthesized by Twist Bioscience. lldRO1-J23117-lldRO2 + B0034 and B0015 were obtained as gBlocks from IDT and these 3 parts were joined together using SOEing (Spliced Overlap Extension) PCR.

References

Zhang, G., Brokx, S., & Weiner, J. H. (2005). Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nature Biotechnology, 24(1), 100–104. doi:10.1038/nbt1174
Team ETH Zurich 2015

@@ Line 8: / Line 8: @@
 ===Design Notes===
 IL12 and YebF was two different parts used by two different iGEM teams. In order to improve the part used by Brazil ([[BBa_K554005]]), <b>we have codon optimized it to make it synthesize compatible and attached a Secretory peptide YebF so that IL12 also get secreted along with YebF</b>
-SO, the whole part contains:
+So, the whole part contains:
 <ul>
 <li>The Promoter and Operator Region: lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]])