Difference between revisions of "Part:BBa K103020:Experience"

Revision as of 13:14, 26 October 2008

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Applications of BBa_K103020

'Hunter and prey' selection system

This part was used as core of prey generator device. It was fused with:

protein A BBa_K103003. Resulting fusion was named His_omega_A
protein Z_SPA-1 BBa_K103004. . Resulting fusion was named His_omega_Z

Both fusions were overexpressed in BL21 strain. Due to ability of protein Z to form aggregates His_omega_Z was purified simply by collecting post-sonication debris. His_omega_A was purified on NiNTA-agarose bead (elution peak in 150 mM imidazole). The both prey protein fusions were added to culture media and used in selection experiments. Details here and here.

User Reviews

UNIQfbc71a2969995c14-partinfo-00000000-QINU UNIQfbc71a2969995c14-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K103020===
+==='Hunter and prey' selection system===
+This part was used as core of prey generator device. It was fused with:
+*protein A [[Part:BBa_K103003 | BBa_K103003]]. Resulting fusion was named His_omega_A
+*protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. . Resulting fusion was named His_omega_Z
+Both fusions were overexpressed in BL21 strain. Due to ability of protein Z to form aggregates His_omega_Z was purified simply by collecting post-sonication debris.
+His_omega_A was purified on NiNTA-agarose bead (elution peak in 150 mM imidazole).
+The both prey protein fusions were added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]].
 ===User Reviews===