Difference between revisions of "Part:BBa K3137000"

Revision as of 14:52, 21 October 2019

PrsmH

RsmH can detect the intensity of the fluorescence of gfp(BBa_K3137011).

Usage and Biology

In the case of determining that the combination resistant protein is effective, we chose the constitutive promoter PrsmH to constitutively express the resistant protein in an attempt to verify whether the resistant protein has an effect on cell growth. First, we linked this constitutive promoter PrsmH to gfp(BBa_K3137011), demonstrating the validity of this constitutive promoter. The effect of overexpression of the resistant protein AbpAB(BBa_K3137006) on growth was then verified. The results showed that PrsmH had a good effect, and overexpression of AbpAB had no significant effect on the growth of E. coli BL21.

Green fluorescent of E. coli BL21-pET28a-PrsmH-gfp.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 RsmH can detect the intensity of the fluorescence of gfp([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]).
-[[Image:RsmH.jpeg|400px|thumb|center|Figure1. Green fluorescent of E. coli BL21-pET28a-PrsmH-gfp.]]
 ===Usage and Biology===
+In the case of determining that the combination resistant protein is effective, we chose the constitutive promoter PrsmH to constitutively express the resistant protein in an attempt to verify whether the resistant protein has an effect on cell growth.
+First, we linked this constitutive promoter PrsmH to gfp([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]), demonstrating the validity of this constitutive promoter. The effect of overexpression of the resistant protein AbpAB([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006]) on growth was then verified. The results showed that PrsmH had a good effect, and overexpression of AbpAB had no significant effect on the growth of E. coli BL21.
-File:RsmH.jpeg
+[[Image:RsmH.jpeg|400px|thumb|center|Green fluorescent of E. coli BL21-pET28a-PrsmH-gfp.]]