Difference between revisions of "Part:BBa K103017:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===User Reviews===
 
===User Reviews===
 
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<partinfo>BBa_K103017 AddReview number</partinfo>
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<partinfo>BBa_K103017 AddReview 5</partinfo>
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<I>Username</I>
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<I>Keton</I>
 
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Enter the review inofrmation here.
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We have used this part in fusion with [[Part:BBa_K103003 | BBa_K103003]] and [[Part:BBa_K103004 | BBa_K103004]] to create hunter expression devices:
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*OmpA_alpha_A (compatible prey: His_omega_Z - fusion of [[Part:BBa_K103020 | BBa_K103020]] with [[Part:BBa_K103004 | BBa_K103004]])
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*OmpA_alpha_Z (compatible prey: His_omega_A  - fusion of [[Part:BBa_K103020 | BBa_K103020]] with [[Part:BBa_K103003 | BBa_K103003]])
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*OmpA_alpha negative control device without hunter [[Part:BBa_K103017 | BBa_K103017]]
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*OmpA_alpha_A_omega positive control device containing both beta-lactamase halves [[Part:BBa_K103017 | BBa_K103017]] in fusion with [[Part:BBa_K103003 | BBa_K103003]] and [[Part:BBa_K103012 | BBa_K103012]]
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To check optimal IPTG and ampicilin concentrations we have used OmpA_alpha_A_omega positive control device which should make cells ampicilin resistant. We have obtained following results:
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{| style='border:1px solid gray; border-collapse: collapse;'
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! colspan="7" style='border:1px solid gray; border-collapse: collapse;' | OD<sub>600</sub> of Top10 cultures expressing OmpA_alpha_A_omega
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|-
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! rowspan="2" valign="top" style='border:1px solid gray; border-collapse: collapse;' | Ampicilin concentration (ug /ml)
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! colspan="6" style='border:1px solid gray; border-collapse: collapse;' | IPTG Concentration (uM)
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! style='border:1px solid gray; border-collapse: collapse;' | 0
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! style='border:1px solid gray; border-collapse: collapse;' | 0.1
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! style='border:1px solid gray; border-collapse: collapse;' | 0.25
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! style='border:1px solid gray; border-collapse: collapse;' | 0.5
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! style='border:1px solid gray; border-collapse: collapse;' |  0.75
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! style='border:1px solid gray; border-collapse: collapse;' |  1
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|-
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! style='border:1px solid gray; border-collapse: collapse;' |  25
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| style='border:1px solid gray; border-collapse: collapse;' | 1.558
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| style='border:1px solid gray; border-collapse: collapse;' | 1.469
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| style='border:1px solid gray; border-collapse: collapse;' | 1.587
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| style='border:1px solid gray; border-collapse: collapse;' | 1.49
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| style='border:1px solid gray; border-collapse: collapse;' | 1.566
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| style='border:1px solid gray; border-collapse: collapse;' | 1.311
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|-
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! style='border:1px solid gray; border-collapse: collapse;' | 50
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| style='border:1px solid gray; border-collapse: collapse;' | 1.425
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| style='border:1px solid gray; border-collapse: collapse;' | 1.435
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| style='border:1px solid gray; border-collapse: collapse;' | 1.524
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| style='border:1px solid gray; border-collapse: collapse;' | 1.055
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| style='border:1px solid gray; border-collapse: collapse;' | 0.920
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| style='border:1px solid gray; border-collapse: collapse;' | 0.935
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|-
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! style='border:1px solid gray; border-collapse: collapse;' | 75
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| style='border:1px solid gray; border-collapse: collapse;' | 1.09
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| style='border:1px solid gray; border-collapse: collapse;' | 0.989
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| style='border:1px solid gray; border-collapse: collapse;' | 1.447
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| style='border:1px solid gray; border-collapse: collapse;' | 0.971
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| style='border:1px solid gray; border-collapse: collapse;' | 0.951
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| style='border:1px solid gray; border-collapse: collapse;' | 0.992
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|-
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! style='border:1px solid gray; border-collapse: collapse;' | 100
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| style='border:1px solid gray; border-collapse: collapse;' | 0.09
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| style='border:1px solid gray; border-collapse: collapse;' | 0.685
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| style='border:1px solid gray; border-collapse: collapse;' | 1.378
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| style='border:1px solid gray; border-collapse: collapse;' | 1.078
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| style='border:1px solid gray; border-collapse: collapse;' | 0.977
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| style='border:1px solid gray; border-collapse: collapse;' | 0.992
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|-
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Revision as of 11:42, 25 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K103017

User Reviews

UNIQ8625b7a8510f85ad-partinfo-00000000-QINU

•••••

Keton

We have used this part in fusion with BBa_K103003 and BBa_K103004 to create hunter expression devices:

To check optimal IPTG and ampicilin concentrations we have used OmpA_alpha_A_omega positive control device which should make cells ampicilin resistant. We have obtained following results:

OD600 of Top10 cultures expressing OmpA_alpha_A_omega
Ampicilin concentration (ug /ml) IPTG Concentration (uM)
0 0.1 0.25 0.5 0.75 1
25 1.558 1.469 1.587 1.49 1.566 1.311
50 1.425 1.435 1.524 1.055 0.920 0.935
75 1.09 0.989 1.447 0.971 0.951 0.992
100 0.09 0.685 1.378 1.078 0.977 0.992


UNIQ8625b7a8510f85ad-partinfo-00000002-QINU