Difference between revisions of "Part:BBa K3137014"
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The respond gene circuit is composed of an inducible promoter PputA([https://parts.igem.org/Part:BBa_K3137002# BBa_K3137002]) and anti-phage protein genes of the incubation period (about 5 min), and an inducible promoter PglcF ([https://parts.igem.org/Part:BBa_K3137003# BBa_K3137003]) and a toxic protein gene protegrin-1([https://parts.igem.org/Part:BBa_K62800# BBa_K62800]) of the burst period (about 20 min).
 
The respond gene circuit is composed of an inducible promoter PputA([https://parts.igem.org/Part:BBa_K3137002# BBa_K3137002]) and anti-phage protein genes of the incubation period (about 5 min), and an inducible promoter PglcF ([https://parts.igem.org/Part:BBa_K3137003# BBa_K3137003]) and a toxic protein gene protegrin-1([https://parts.igem.org/Part:BBa_K62800# BBa_K62800]) of the burst period (about 20 min).
  
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When <i>E. coli</i> is infected by a phage, respond gene circuit is induced to express. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to express the resistant proteins ([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006])([https://parts.igem.org/Part:BBa_K3137005# BBa_K3137005]).
+
When <i>E. coli</i> is infected by a phage, respond gene circuit is induced to express. When a phage infects <i>E. coli</i> into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to express the resistant proteins ([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006])([https://parts.igem.org/Part:BBa_K3137005# BBa_K3137005]).
  
  

Revision as of 09:11, 21 October 2019


responce circuit

Respond circuit2.png

Usage and Biology

The respond gene circuit is composed of an inducible promoter PputA(BBa_K3137002) and anti-phage protein genes of the incubation period (about 5 min), and an inducible promoter PglcF (BBa_K3137003) and a toxic protein gene protegrin-1(BBa_K62800) of the burst period (about 20 min).

When E. coli is infected by a phage, respond gene circuit is induced to express. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to express the resistant proteins (BBa_K3137006)(BBa_K3137005).


If the resistant proteins successfully resist phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that phages will continue to infect bacteria, when it comes to the burst period (around 20 min) of phage infection, the inducible promoter PglcF will activate the expression of downstream toxic protein gene so that the bacteria will lyse cells before the assembly of phages.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1280
    Illegal EcoRI site found at 2048
    Illegal PstI site found at 1313
    Illegal PstI site found at 2705
    Illegal PstI site found at 3785
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1280
    Illegal EcoRI site found at 2048
    Illegal PstI site found at 1313
    Illegal PstI site found at 2705
    Illegal PstI site found at 3785
    Illegal NotI site found at 4987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1280
    Illegal EcoRI site found at 2048
    Illegal BglII site found at 1619
    Illegal BglII site found at 2679
    Illegal BamHI site found at 481
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1280
    Illegal EcoRI site found at 2048
    Illegal PstI site found at 1313
    Illegal PstI site found at 2705
    Illegal PstI site found at 3785
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1280
    Illegal EcoRI site found at 2048
    Illegal PstI site found at 1313
    Illegal PstI site found at 2705
    Illegal PstI site found at 3785
    Illegal NgoMIV site found at 4391
  • 1000
    COMPATIBLE WITH RFC[1000]