Difference between revisions of "Part:BBa K3137011"
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The gfp can express green fluorescent protein, which can be used as a marker gene in the process to report the number of cells.
 
The gfp can express green fluorescent protein, which can be used as a marker gene in the process to report the number of cells.
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===Usage and Biology===
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When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to (([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006])([https://parts.igem.org/Part:BBa_K3137005# BBa_K3137005])) display green fluorescence.
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[[Image:PputA.png|500px|thumb|center|Characterization of PputA]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:38, 21 October 2019


gfp

The gfp can express green fluorescent protein, which can be used as a marker gene in the process to report the number of cells.

Usage and Biology

When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to ((BBa_K3137006)(BBa_K3137005)) display green fluorescence.


Characterization of PputA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644