Difference between revisions of "Part:BBa K3110007"
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L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and  lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
 
L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and  lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
  
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3110007 parameters</partinfo>
 
<partinfo>BBa_K3110007 parameters</partinfo>
 
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<h1>Characterization of the Part</h1>
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[[File:T--IISER Tiruapti--lldD.png|thumb|center|700px|Figure 1. Relative Expression of sfGFP under the control of lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]]) and Constitutive Promoter with Strong RBS along with Medium P-Strong RBS-lldD.]]

Revision as of 06:26, 21 October 2019


Medium Promoter Strong RBS lldD

This construct has L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and a strong RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 639


Characterization of the Part

Figure 1. Relative Expression of sfGFP under the control of lldRO1-J23117-lldRO2 (Part:BBa_K1847008) and Constitutive Promoter with Strong RBS along with Medium P-Strong RBS-lldD.