Difference between revisions of "Part:BBa K2924051"

Revision as of 17:12, 20 October 2019

Leaderless 'TesA

Rhamnose-inducible promoter (similar to BBa_K914003) and RBS* (BBa_K2924009¹) expressing the thioesterase tesA (BBa_K1472601)and a double terminator (BBa_B0015). Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2924051 short</partinfo>
-placeholder
+Rhamnose-inducible promoter (similar to <html><a href="https://parts.igem.org/Part:BBa_K914003">BBa_K914003</a>) and RBS* (<a href="https://parts.igem.org/Part:BBa_K2924009">BBa_K2924009</a><sup>1</sup>) expressing the thioesterase tesA (<a href="https://parts.igem.org/Part:BBa_K1472601">BBa_K1472601</a>)and a double terminator (<a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>).
+</html>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part contains the rhamnose-inducible promoter P<sub>rha</sub> described by Kelly <i>et al.</i> (2018)<sup>-1<sup> which was further modified by Behle <i>et al.</i> (2019)<sup>0<sup>, and is similar to <a href="https://parts.igem.org/Part:BBa_K914003">(BBa_K914003)</a>, the RBS* <a href="https://parts.igem.org/Part:BBa_K2924009">(BBa_K2924009)</a><sup>1</sup>, a thioesterase from <i>Escherichia coli</i> <a href="https://parts.igem.org/Part:BBa_K1472601">(BBa_K1472601)</a> and a double terminator (BBa_B0015).
+In general, all basic parts used here (with the exception of ‘TesA) were optimized for <i>Synechocystis sp.</i> PCC 6803, but function efficiently in both <i>E. coli</i> and <i>Synechocystis sp.</i>.
+This thioesterase lacks a leader peptide sequence, which is the N-terminal 26-amino acid signal peptide sequence. ’TesA is located in the cytosol of <i>E.coli</i>, where it hydrolyzes the bond of fatty acyl to acyl-carrier protein (ACP) or Coenzyme A (CoA). This results in a free fatty acids and either ACP or CoA.
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