Difference between revisions of "Part:BBa K3187045"

Latest revision as of 08:56, 20 October 2019

P22 Bacteriophage Coat and Scaffolding Protein (tetR/tetA promotor)

This part is a generator for the P22 bacteriophage capsid containing both capsomers coat protein and scaffold protein under the control of a TetR/tetA promoter. Expression can be induced by addition of anhydrotetracyclin (AHT). This part was used together with BBa_K3187044 for the dual expression of the P22 capsid with different ratios of LPETGG-tagged coat protein to untagged coat protein. The potential of fine tuning the expression levels of both parts was tested via BBa_K3187011.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal PstI site found at 1931
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal NheI site found at 1936
Illegal PstI site found at 1931
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal BamHI site found at 1389
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal PstI site found at 1931
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal PstI site found at 1931
Illegal NgoMIV site found at 1783
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 <html>
 <p>
-This part is a generator for the P22 bacteriophage capsid containing the both capsomers coat protein and scaffold protein under the control of a TetR/tetA promoter. Expression can be induced by addition of anhydrotetracyclin (AHT). This part was used together with <a
+This part is a generator for the P22 bacteriophage capsid containing both capsomers coat protein and scaffold protein under the control of a TetR/tetA promoter. Expression can be induced by addition of anhydrotetracyclin (AHT). This part was used together with <a
                      href=" https://parts.igem.org/Part:BBa_K3187044" target="_blank">BBa_K3187044</a> for the dual expression of the P22 capsid with different ratios of LPETGG-tagged coat protein to untagged coat protein. The potential of fine tuning the expression levels of both parts was tested via <a
                      href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>.
@@ Line 15: / Line 15: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<h2><span class='h3bb'>Sequence and Features</span></h2>
 <partinfo>BBa_K3187045 SequenceAndFeatures</partinfo>