Difference between revisions of "Part:BBa K3037008"
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== Overview == | == Overview == | ||
| − | The TU Dresden 2019 team design this fusion protein in order to increase the expression of HRP. In order to make it the RFC 25 standard was used | + | The TU Dresden 2019 team design this fusion protein in order to increase the expression of HRP. In order to make it the RFC 25 standard was used [https://2019.igem.org/Team:TU_Dresden/Parts (more information).] |
HRP was inserted into the pSB1C3 vector for transformation and expressed in <span style="font-style: italic;">Escherichia coli</span>. | HRP was inserted into the pSB1C3 vector for transformation and expressed in <span style="font-style: italic;">Escherichia coli</span>. | ||
Revision as of 15:21, 19 October 2019
MBP+HRP
| MBP+HRP | |
|---|---|
| Function | Expression |
| Use in | Escherichia coli |
| RFC standard | Freiburg RFC25 standard |
| Backbone | pSB1C3 |
| Submitted by | Team: TU_Dresden 2019 |
Contents
Overview
The TU Dresden 2019 team design this fusion protein in order to increase the expression of HRP. In order to make it the RFC 25 standard was used (more information).
HRP was inserted into the pSB1C3 vector for transformation and expressed in Escherichia coli.
The HRP was made from BBa_K1800002 but adapted to the RFC 25 standard
Characterization
Outline
1) Expression in pOCC97, growing curve (BBa_3037000)
Experiments in Detail
Expression in BBa_K3037000 (pOCC97)
Sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1357
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 381
Illegal BglII site found at 1599
Illegal XhoI site found at 1683 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 79