Difference between revisions of "Part:BBa K3015004"
| Line 5: | Line 5: | ||
The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005|BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. | The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005|BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. | ||
The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. | The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. | ||
| − | + | To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Regsitry-Page of the described Composite Part [[Part:BBa_K3015007|BBa_K3015007]] | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 11:55, 19 October 2019
Theophylline_Riboswitch (transcriptional)
The iGEM Team BOKU-Vienna improved the Part BBa_K784005 by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Regsitry-Page of the described Composite Part BBa_K3015007
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]