Difference between revisions of "Part:BBa K2922038"

Revision as of 21:04, 18 October 2019

The colicin-N operon under T7 promoter control with a gfasPurple chromoprotein reporter

Summary

This is a composite part consist of a T7 promoter (BBa_K525998), CDS of colicin-N (BBa_K2922027), CDS of colicin-N immunity protein (BBa_K2922028), CDS of lysis protein (BBa_K2922029) and a gfasPurple chromoprotein reporter(BBa_K1033917) . Each CDS has an RBS (BBa_B0034) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. The gfasPurple chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our “aggressive” design.

Ientification

Fig.2 The growth curve of E coli BL21 strain which contained BBa_K2922038 carried by pSB1C3. OD600, OD577 and OD458 is detected to show the concentration of gfasPurple and amajLime chromoproteins.

Fig.3 The co-cultural growth curve of E coli BL21 strains which contained BBa_K2922038 or BBa_K1033914. OD600, OD577 and OD458 is detected to specifically track each strain`s growth status.IPTG is added in 2h to induced the expression of colicin.

Fig.4 The co-cultural growth curve of E coli BL21 strains which contained BBa_K2922038 or BBa_K1033914. OD600, OD577 and OD458 is detected to specifically track each strain`s growth status. These is result of control group which IPTG is not added.

Fig.5 The comparison of OD600 between experimental and control group.