Difference between revisions of "Part:BBa K2934006:Design"
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The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1]. | The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1]. | ||
| + | Primers for isolation of the gene (with RFC[10] suffix and prefix): | ||
| + | fwd: 5'-CGCTTCTAGATGTTTGCAAAACGATTCAAAACCTC-3' | ||
| + | |||
| + | rev: 5'-CGCTACTAGTATAAGAACATAATATCATCTC-3' | ||
===Source=== | ===Source=== | ||
Revision as of 13:48, 18 October 2019
AmyE - Invertase-Histag for Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 989
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1294
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 595
Illegal BsaI.rc site found at 937
Illegal SapI.rc site found at 1197
Design Notes
The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1].
Primers for isolation of the gene (with RFC[10] suffix and prefix):
fwd: 5'-CGCTTCTAGATGTTTGCAAAACGATTCAAAACCTC-3'
rev: 5'-CGCTACTAGTATAAGAACATAATATCATCTC-3'
Source
Invertase - BBa_K2934001
AmyE signal peptide - BBa_K2273023
linker - BBa_K2934005