Difference between revisions of "Part:BBa K2934008:Design"

(→‎Design Notes)
(→‎Source)
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This part is made up of several basic parts:
 
This part is made up of several basic parts:
−
pKatA - BBa_K2934002
 
−
3' UTR - BBa_K2934007
 
−
LacI - BBa_K143033
 
−
5' UTR - BBa_K2934003
 
−
pLac - BBa_K143015
 
−
GOx with secretory signal peptide AmyE - BBa_K2934004
 
  
 +
pKatA - [https://parts.igem.org/Part:BBa_K2934002 BBa_K2934002]
 +
 +
3' UTR - [https://parts.igem.org/Part:BBa_K2934007 BBa_K2934007]
 +
 +
LacI - [https://parts.igem.org/Part:BBa_K143033 BBa_K143033]
 +
 +
5' UTR - [https://parts.igem.org/Part:BBa_K2934003 BBa_K2934003]
 +
 +
pLac - [https://parts.igem.org/Part:BBa_K143015 BBa_K143015]
 +
 +
GOx with secretory signal peptide AmyE - [https://parts.igem.org/Part:BBa_K2934004 BBa_K2934004]
  
 
===References===
 
===References===

Revision as of 13:21, 18 October 2019


pKatA-LacI-pLac-Glucose Oxidase-Histag for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1564
    Illegal BsaI site found at 1895
    Illegal BsaI.rc site found at 1841
    Illegal BsaI.rc site found at 2666


Design Notes

In our lab, we devided this part into two DNA fragments, with 40 non-coding DNA spacer segment between them (that we used as a homologic region to fuse both DNA fragments via Gibson Assembly). The spacer was located between 5' UTR and the following promoter.

Primers for isolation of the gene (with RFC[10] suffix and prefix):

fwd: 5'-GGCCGCTTCTAGAGATAACTATTTT-3'

rev: 5'-GTATTAGTGGTGATGATGGTGATGTC-3'

Source

This part is made up of several basic parts:

pKatA - BBa_K2934002

3' UTR - BBa_K2934007

LacI - BBa_K143033

5' UTR - BBa_K2934003

pLac - BBa_K143015

GOx with secretory signal peptide AmyE - BBa_K2934004

References