Difference between revisions of "Part:BBa K2934006:Design"

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linker - [https://parts.igem.org/Part:BBa_K2934005 BBa_K2934005]
 
linker - [https://parts.igem.org/Part:BBa_K2934005 BBa_K2934005]
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The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (<em>B. subtilis</em> Secretory Protein Expression System) [1].
 
  
 
===References===
 
===References===
  
 
[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system
 
[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system

Revision as of 16:34, 17 October 2019


AmyE - Invertase-Histag for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1294
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 595
    Illegal BsaI.rc site found at 937
    Illegal SapI.rc site found at 1197


Design Notes

The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1].


Source

Invertase - BBa_K2934001

AmyE signal peptide - BBa_K2273023

linker - BBa_K2934005

References

[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system