Difference between revisions of "Part:BBa K2961000"

Latest revision as of 01:41, 17 October 2019

CFTR Mutant Gene Segment (rs75389940_G)

This part encodes for a sequence that is a segment that is specific to the CFTR gene, but specifically SNP variant rs75389940_G, which is a common allele in the Middle East responsible for the prevalence of cystic fibrosis.

This part has been designed to strategically be a copy of a segment of the CFTR gene that is close to the PAM site for Cpf1, so that it may be used for targeting through gRNA design.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2961000 short</partinfo>
-This part encodes for a guide RNA that can be used with Cpf1. The sequence includes a crRNA repeat, known as the fixed loop, to allow Lab Cas12a to recognize the gRNA (different manufacturers may produce Cas12a that are optimized for recognizing different fixed loops; the sequence posted here is optimized for NEB Cas12a). The sequence also includes a segment that is specific to the CFTR gene, specifically the SNP variant rs75389940_G, which is a common allele in the Middle East responsible for the prevalence of cystic fibrosis.
+This part encodes for a sequence that is a segment that is specific to the CFTR gene, but specifically SNP variant rs75389940_G, which is a common allele in the Middle East responsible for the prevalence of cystic fibrosis.
-This part may be used to simply manufacture this gRNA. It can be used in conjunction with template DNA extracted from patients or synthesized in a lab to allow Cas12a to recognize and bind to the mutated CFTR gene. This may then be paired with further downstream effects (such as Cas12a activity causing fluorescence of some kind) to detect DNA samples that have the genetic disease.
+This part has been designed to strategically be a copy of a segment of the CFTR gene that is close to the PAM site for Cpf1, so that it may be used for targeting through gRNA design.
 <!-- Add more about the biology of this part here