Difference between revisions of "Part:BBa K2951000"
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This part contains the original sequence of Influenza A nucleocapsid protein(NP) obtained from NCBI. | This part contains the original sequence of Influenza A nucleocapsid protein(NP) obtained from NCBI. | ||
| + | ==Characterizaiton== | ||
| + | This part was characterized by CSMU_Taiwan 2019.Because of its stringent sequence specificity, tobacco etch virus (TEV) protease emerges as a useful reagent with wide application in the cleavage of recombinant fusion proteins. Thus, we have chosen TEV protease(sequence from [https://parts.igem.org/Part:BBa_K1319004 BBa_K1319004]) to remove the fusion protein in our another part [https://parts.igem.org/Part:BBa_K2951008 BBa_K2951008] and be characterized. | ||
| + | |||
| + | ===Small scale production=== | ||
| + | ====Cultivations and Induction of protein expression==== | ||
| + | |||
| + | ===Large scale production=== | ||
| + | ====Cultivations and Induction of protein expression==== | ||
| + | |||
| + | ====Protein solubility analysis==== | ||
| + | |||
| + | ===Purification and Dialysis=== | ||
| + | |||
| + | ===Enzyme activity assay=== | ||
===References=== | ===References=== | ||
Revision as of 18:13, 12 October 2019
Influenza A virus nucleocapsid protein
Contents
Short Description
This part contains the original sequence of Influenza A nucleocapsid protein(NP) obtained from NCBI.
Characterizaiton
This part was characterized by CSMU_Taiwan 2019.Because of its stringent sequence specificity, tobacco etch virus (TEV) protease emerges as a useful reagent with wide application in the cleavage of recombinant fusion proteins. Thus, we have chosen TEV protease(sequence from BBa_K1319004) to remove the fusion protein in our another part BBa_K2951008 and be characterized.
Small scale production
Cultivations and Induction of protein expression
Large scale production
Cultivations and Induction of protein expression
Protein solubility analysis
Purification and Dialysis
Enzyme activity assay
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 477
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 238