Difference between revisions of "Part:BBa K1033933"
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This part is useful as a reporter. | This part is useful as a reporter. | ||
Revision as of 13:01, 11 October 2019
asPink, pink chromoprotein
This chromoprotein from the coral Anemonia sulcata, asPink (also known as asCP or asFP595), naturally exhibits strong color when expressed. The protein has an absorption maximum at 572 nm giving it a pink/purple color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein asPink has significant sequence homologies with proteins in the GFP family.
Important: This part is not available in the registry yet, however, the same part is available from the registry with the standard RBS B0034: BBa_K1033927.
Contribution
Group: Linkoping_Sweden iGEM 2019
Author: Andreas Holmqvist and Leo Juhlin
Summary: We tested the oxygen dependency of the chromophore protein expression in E.coli BL21(DE3) cells and then measured the absorbance of the cells with an UV-spectrophotometer.
Documentation:
The expression system used contained the following parts:
- the BBa_B0034 ribosome binding site
- the BBa_J23110 Constitutive promotor
Usage and Biology
This part is useful as a reporter.
asPink does not exist as CDS only but only as RBS-asPink (BBa_K1033927) and J23110-RBS-spisPink (BBa_K1033926).
iGEM2013 Uppsala: Expression of asPink in E. coli DH5alpha by promoter J23110 from medium copy plasmid pSB3K3 (left) or high copy plasmid pSB1K3 (right).
Source
The protein was first extracted and characterized by Lukyanov et. al. 2000 under the name asFP595 (GenBank: AAG02385.1). This version is codon optimized for E coli by Genscript.
References
[http://www.jbc.org/content/275/34/25879.short]Lukyanov, Konstantin A., et al. "Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog." Journal of Biological Chemistry 275.34 (2000): 25879-25882.
[http://www.jbc.org/content/280/4/2401.short]Wilmann, Pascal G., et al. "Variations on the GFP Chromophore - A Polypeptide Fragmentation Within The Chromophore Revealed In The 2.1-Å Crystal Structure Of A Nonfluorescent Chromoprotein From Anemonia Sulcata." Journal of Biological Chemistry 280.4 (2005): 2401-2404.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]