Difference between revisions of "Plasmid backbones"
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|align='center' width=150px|'''[[Plasmids/Other|Non-BioBrick plasmids]] | |align='center' width=150px|'''[[Plasmids/Other|Non-BioBrick plasmids]] | ||
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|align='center' width=150px|'''[[Plasmids/Archive|Archive]] | |align='center' width=150px|'''[[Plasmids/Archive|Archive]] | ||
Revision as of 18:49, 2 September 2008
| Help! |
| Part assembly |
| Assembly of protein fusions |
| Part measurement |
| System operation |
| Building new BioBrick vectors |
| Non-BioBrick plasmids |
| Archive |
Plasmids are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. Plasmid DNA is easily purified from cells, manipulated using common lab techniques and incorporated into cells. Most BioBrick parts in the Registry are maintained and propagated on plasmids. Thus, construction of BioBrick parts, devices and systems usually requires working with plasmids.
There are many different types of plasmids and plasmid parts in the Registry. Here we've tried to collect all plasmids and plasmid parts into one place for easy browsing and retrieval.
- Assembly: Are you trying to assemble two BioBrick parts together? There are a set of plasmids available designed to make assembly of BioBrick parts easier.
- Assembly of protein fusions: Are you trying to do an in-frame assembly of two protein domains? There are a set of plasmids available designed to make assembly of protein domains easier.
- Measurement: Are you interested in measuring the behavior of a BioBrick promoter, ribosome binding site, or transcriptional inverter? Jason Kelly has designed various plasmids to streamline the process of part characterization so that you can compare your results to those that others have gotten.
- Operation: Oftentimes, it pays to use a different set of plasmids for operating or running your BioBrick device or system than you use for assembly. For example, high copy plasmids are great for getting good DNA yields when doing plasmid purification which is helpful when assembling parts. However, some BioBrick devices and systems consume too many resources when operated on a high copy plasmid and significantly impact cell growth. In those cases, you might want to switch to a low or medium copy plasmid.
- Building new BioBrick vectors: Sometimes there just isn't a plasmid already available that has the replication origin and antibiotic resistance marker that you want. In that case, you might need to build a new BioBrick vector. But you don't need to start from scratch. Here are a set of BioBrick parts that might help you in building a new BioBrick vector.
- Non-BioBrick plasmids: Some Registry contributors have built BioBrick plasmids that don't strictly comply with the BioBrick assembly standard, but still might be useful to you. Check out those plasmids here.
- Archive: Older plasmids that have been replaced by new versions of BioBrick plasmids.
(This page is a work in progress. Thank you for your patience. If you'd like to contribute, contact [http://openwetware.org/wiki/User:Reshma_P._Shetty Reshma Shetty])