Difference between revisions of "Part:BBa K2588050"
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| − | + | <p>Using the terminator <a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>, the constitutive promotor <a href="https://parts.igem.org/Part:BBa_J23106">BBa_J23106</a>, and the RnaG120 based terminator <a href="https://parts.igem.org/Part:BBa_K2588004">BBa_K2588004</a> the function of a promotor cloned downstream of this sequence in reverse direction can invert the promotors function.</p> | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 03:55, 18 October 2018
RnaG120-based NOT gate to invert promotor function
Using the terminator BBa_B0015, the constitutive promotor BBa_J23106, and the RnaG120 based terminator BBa_K2588004 the function of a promotor cloned downstream of this sequence in reverse direction can invert the promotors function.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 144
Illegal NheI site found at 167 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]