Difference between revisions of "Part:BBa K2656201"

Latest revision as of 02:35, 18 October 2018

Briobrickation part

This part is a transcriptional unit including:

In addition, this part is flanked upstream with the sequence ggagtgagacg and downstream with the sequence cgtctcagtca. These sequences include a BsmbI Type IIS endonuclease recognition site and the appropriate resulting sticky ends to accept a GoldenBraid 3.0 alpha1 insert.

This construction has been ligated into the BB backbone pSB1C3, so designing the pBioBrickator plasmid. Thus, in this BB compatible vector, BBa_K2656201 acts as a selection marker to carry out the red-white screening (Figure 1) while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units.

Figure 1. Example of Biobrickation reaction and red-white(green in this case) selection.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 18
Illegal NheI site found at 41
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 627
Illegal AgeI site found at 739
1000
COMPATIBLE WITH RFC[1000]

@@ Line 15: / Line 15: @@
 In addition, this part is flanked upstream with the sequence ggagtgagacg and downstream with the sequence cgtctcagtca. These sequences include a BsmbI Type IIS endonuclease recognition site and the appropriate resulting sticky ends to accept a GoldenBraid 3.0 alpha1 insert.
-This construction has been ligated into the BB backbone pSB1C3, so designing the [https://parts.igem.org/Part:http://2018.igem.org/Team:Valencia_UPV/Part_Collection#pls pBioBrickator plasmid]. Thus, in this BB compatible vector, BBa_K265620 acts as a selection marker to carry out the red-white screening (Figure 1) while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units.
+This construction has been ligated into the BB backbone pSB1C3, so designing the [https://parts.igem.org/Part:http://2018.igem.org/Team:Valencia_UPV/Part_Collection#pls pBioBrickator plasmid]. Thus, in this BB compatible vector, BBa_K2656201 acts as a selection marker to carry out the red-white screening (Figure 1) while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units.
 [[File:T--Valencia_UPV--P_Biobrickator.jpeg|250px|thumb|center|alt=domestication.|Figure 1. Example of Biobrickation reaction and red-white(green in this case) selection.]]