Difference between revisions of "Part:BBa K2656201"
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This construction has been ligated into the BB backbone pSB1C3, so designing the [https://parts.igem.org/Part:http://2018.igem.org/Team:Valencia_UPV/Part_Collection#pls pBioBrickator plasmid]. Thus, in this BB compatible vector, BBa_K265620 acts as a selection marker to carry out the red-white screening (Figure 1) while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units. | This construction has been ligated into the BB backbone pSB1C3, so designing the [https://parts.igem.org/Part:http://2018.igem.org/Team:Valencia_UPV/Part_Collection#pls pBioBrickator plasmid]. Thus, in this BB compatible vector, BBa_K265620 acts as a selection marker to carry out the red-white screening (Figure 1) while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units. | ||
− | [[File:T--Valencia_UPV--P_Biobrickator.jpeg|250px|thumb| | + | [[File:T--Valencia_UPV--P_Biobrickator.jpeg|250px|thumb|right|alt=domestication.|Figure 1. Example of Biobrickation reaction and red-white(green in this case) selection.]] |
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Revision as of 02:34, 18 October 2018
Briobrickation part
This part is a transcriptional unit including:
- BBa_K2656004 (GoldenBraid 3.0. version of J23106 promoter)
- BBa_K2656009 (GoldenBraid 3.0. version of B0030 RBS)
- BBa_K2656014 (GoldenBraid 3.0. version of E1010 CDS, mRFP1)
- BBa_K2656026 (GoldenBraid 3.0. version of B0015 terminator)
In addition, this part is flanked upstream with the sequence ggagtgagacg and downstream with the sequence cgtctcagtca. These sequences include a BsmbI Type IIS endonuclease recognition site and the appropriate resulting sticky ends to accept a GoldenBraid 3.0 alpha1 insert.
This construction has been ligated into the BB backbone pSB1C3, so designing the pBioBrickator plasmid. Thus, in this BB compatible vector, BBa_K265620 acts as a selection marker to carry out the red-white screening (Figure 1) while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 18
Illegal NheI site found at 41 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 627
Illegal AgeI site found at 739 - 1000COMPATIBLE WITH RFC[1000]