Difference between revisions of "Part:BBa K2675040:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
BBa_J23102 Promoter + RBS (BBa_K2675010) + AimR-Phi3T coding sequence (BBa_K2675000) + ECK120029600 Terminator (BBa_K2486006).
+
[[Part:BBa_J23102|BBa_J23102]] constitutive strong Promoter + synthetic RBS ([[Part:BBa_K2675010|BBa_K2675010]]) + AimR of phage phi3T coding sequence ([[Part:BBa_K2675000|BBa_K2675000]]) + ECK120029600 strong Terminator ([[Part:BBa_K2486006|BBa_K2486006]])
  
 
===Source===
 
===Source===
  
−
DNA synthesis
+
DNA synthesis & Assembly
  
 
===References===
 
===References===
 
[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.
 
[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.
 +
 +
[2] Maio A, Brandi L, Donadio S, Gualerzi CO. The Oligopeptide Permease Opp Mediates Illicit Transport of the Bacterial P-site Decoding Inhibitor GE81112. Antibiotics (Basel) (2016) 5, 17.

Latest revision as of 00:51, 18 October 2018


AimR of phage phi3T expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_J23102 constitutive strong Promoter + synthetic RBS (BBa_K2675010) + AimR of phage phi3T coding sequence (BBa_K2675000) + ECK120029600 strong Terminator (BBa_K2486006)

Source

DNA synthesis & Assembly

References

[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.

[2] Maio A, Brandi L, Donadio S, Gualerzi CO. The Oligopeptide Permease Opp Mediates Illicit Transport of the Bacterial P-site Decoding Inhibitor GE81112. Antibiotics (Basel) (2016) 5, 17.