Difference between revisions of "Part:BBa K2684006"
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CsgA fused with SpyTag by 2xGGGGS linker | CsgA fused with SpyTag by 2xGGGGS linker | ||
+ | This part was improved from <a href="https://parts.igem.org/Part:BBa_K1583000">Part BBa_K1583000</a>. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<div> | <div> | ||
<h2>Overview</h2> | <h2>Overview</h2> | ||
− | <p> | + | <p> |
+ | We added a SpyTag sequence which fused after <i>csgA</i> gene, creating <i>csgA-spytag</i> (BBa_K2684006). With SpyTag, CotA laccase can be fixed onto the biofilm by forming a covalent bond SpyTag-SpyCatcher.</p> | ||
<p><img src="https://static.igem.org/mediawiki/2018/b/b9/T--SHSBNU_China--21000.png" style="width:75%"/></image></p> | <p><img src="https://static.igem.org/mediawiki/2018/b/b9/T--SHSBNU_China--21000.png" style="width:75%"/></image></p> | ||
Gene <i>csgA</i> found in the genome of MG1655 wild type is capable of forming biofilm. Using CRISPR, we knocked out gene <i>csgA</i> on MG1655’s genome creating ΔMG1655 strain. The cell ΔMG1655 would then be used as chassis cell. Gene <i>csgA</i> was fused into plasmid pET28a.</p> | Gene <i>csgA</i> found in the genome of MG1655 wild type is capable of forming biofilm. Using CRISPR, we knocked out gene <i>csgA</i> on MG1655’s genome creating ΔMG1655 strain. The cell ΔMG1655 would then be used as chassis cell. Gene <i>csgA</i> was fused into plasmid pET28a.</p> |
Revision as of 21:27, 17 October 2018
CsgA-SpyTag
CsgA fused with SpyTag by 2xGGGGS linker This part was improved from <a href="https://parts.igem.org/Part:BBa_K1583000">Part BBa_K1583000</a>.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Overview
We added a SpyTag sequence which fused after csgA gene, creating csgA-spytag (BBa_K2684006). With SpyTag, CotA laccase can be fixed onto the biofilm by forming a covalent bond SpyTag-SpyCatcher.