Difference between revisions of "Part:BBa K2841503"
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A dCAS9 (<bbpart>BBa_K2841510</bbpart>) gRNA (<bbpart>BBa_K2841514</bbpart>) capable of repressing the LacIq promoter (<bbpart>BBa_K2841517</bbpart>) in response to [http://2018.igem.org/Team:Warwick/Legiolysin Legionella Hemolysin] encoding mRNA. Recognises the Alpha (<bbpart>BBa_K2841531</bbpart>) imitating motif.
 
A dCAS9 (<bbpart>BBa_K2841510</bbpart>) gRNA (<bbpart>BBa_K2841514</bbpart>) capable of repressing the LacIq promoter (<bbpart>BBa_K2841517</bbpart>) in response to [http://2018.igem.org/Team:Warwick/Legiolysin Legionella Hemolysin] encoding mRNA. Recognises the Alpha (<bbpart>BBa_K2841531</bbpart>) imitating motif.
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This part shows 2.1-2.5-fold upregulation upon induction of activity.
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https://static.igem.org/mediawiki/parts/9/94/T--Warwick--2018-Alpha-Graphs.png
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[https://parts.igem.org/File:T--Warwick--2018-Legiolysin-Constructs-AlphaGamma.xlsx Raw Data]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:57, 17 October 2018


Legionella Hemolysin Detector

A dCAS9 (BBa_K2841510) gRNA (BBa_K2841514) capable of repressing the LacIq promoter (BBa_K2841517) in response to [http://2018.igem.org/Team:Warwick/Legiolysin Legionella Hemolysin] encoding mRNA. Recognises the Alpha (BBa_K2841531) imitating motif.

This part shows 2.1-2.5-fold upregulation upon induction of activity.

T--Warwick--2018-Alpha-Graphs.png

Raw Data

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 174
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]