Difference between revisions of "Part:BBa K2656121"

 
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<partinfo>BBa_K2656121 short</partinfo>
 
<partinfo>BBa_K2656121 short</partinfo>
  
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Transcriptional unit created with a Golden Gate reaction using BsaI endonuclease
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Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).
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[[File:T--Valencia_UPV_TU_UPV2018.png|500px|thumb|none|alt=TU.|Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator]]
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This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
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<html>
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<ul>
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<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 BBa_J23106] constitutive  promoter in its Golden Braid compatible version. Promoter T7 leads into a high protein expression. <html></li>
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<li></html>[https://parts.igem.org/Part:BBa_BBa_K2656011 BBa_K2656011]: the [https://parts.igem.org/Part:BBa_B0034 B0034] low ribosome biding site in its Golden Braid compatible version <html></li>
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<li></html> [https://parts.igem.org/Part:BBa_K2656017 BBa_K2656017]: the AraC sequence in its Golden Braid standardized version <html></li>
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<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
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</ul> </html>
  
  

Revision as of 20:25, 17 October 2018


AraC constitutive transcriptional unit

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

TU.
Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656004: the BBa_J23106 constitutive promoter in its Golden Braid compatible version. Promoter T7 leads into a high protein expression.
  • BBa_K2656011: the B0034 low ribosome biding site in its Golden Braid compatible version
  • BBa_K2656017: the AraC sequence in its Golden Braid standardized version
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]