Difference between revisions of "Part:BBa K2467001"
(→An improved version of this part with a sequence optimization has been designed, the improved part is BBa_K2570020) |
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<span style="font-weight:bold;">From 2018 FJNU-China</span> | <span style="font-weight:bold;">From 2018 FJNU-China</span> | ||
We performed a characterization experiment on the proU and used more accurate experimental methods to obtain experimental data. Our team have added new, high quality experimental characterization data to the wildtype promoter sequence of the E.coli proU. | We performed a characterization experiment on the proU and used more accurate experimental methods to obtain experimental data. Our team have added new, high quality experimental characterization data to the wildtype promoter sequence of the E.coli proU. | ||
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| + | ===User Reviews=== | ||
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| + | [[File:FileT--FJNU-China--line of flourencence proU+GFP 2.png|600px|thumb|center|Figure.(B) Fluorescence levels per OD at different salt concentrations.]] | ||
| + | [[File:FileT--FJNU-China--Fluorescence images_proU+GFP_1.png|600px|thumb|center|Figure.(C) Fluorescence images at different salt concentrations.]] | ||
| + | We set the salt concentration 0mm, 50mm, 100mm, 300mm, 500mm (NaCl). In Fig. 1, at the same OD value, the measured GFP fluorescence value increased with increasing salt concentration. In addition, green fluorescence at different salt concentrations can be visually observed, and it is concluded that an increase in salt concentration before 300mM NaCl enhances the expression of a salt-controlled promoter, an increase in the expression of green fluorescent protein, and an increase in GFP fluorescence value. At a salt concentration of 300 mM NaCl to 500 mM NaCl, the fluorescence intensity at the unit OD value is weakened. We speculate that higher salt concentrations have an adverse effect on cell growth and salt-controlled promoter expression. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:16, 17 October 2018
WT proU promoter
This plasmid contains the wildtype promoter sequence of the Escherichia coli proU operon which functions as an osmolarity sensor. In high salt, the osmolarity regulated RFP expression is induced.
An improved version of this part with a sequence optimization has been designed, the improved part is BBa_K2570020
From 2018 FJNU-China We performed a characterization experiment on the proU and used more accurate experimental methods to obtain experimental data. Our team have added new, high quality experimental characterization data to the wildtype promoter sequence of the E.coli proU.
User Reviews
We set the salt concentration 0mm, 50mm, 100mm, 300mm, 500mm (NaCl). In Fig. 1, at the same OD value, the measured GFP fluorescence value increased with increasing salt concentration. In addition, green fluorescence at different salt concentrations can be visually observed, and it is concluded that an increase in salt concentration before 300mM NaCl enhances the expression of a salt-controlled promoter, an increase in the expression of green fluorescent protein, and an increase in GFP fluorescence value. At a salt concentration of 300 mM NaCl to 500 mM NaCl, the fluorescence intensity at the unit OD value is weakened. We speculate that higher salt concentrations have an adverse effect on cell growth and salt-controlled promoter expression.
User Reviews
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]