Part:BBa_K2570020

proU+B0034+GFP+B0032+mazF

This is an improved version of BBa_K2467001.

We explored the effect of toxin protein expression under the control of the salt-controlled promoter. We set different salt concentrations as experimental variables, and the green fluorescent protein was used as the reporter.

Figure.(A) A schematic diagram of a salt-controlled promoter expressing the toxin protein (mazF) circuit.

Figure.(B) Fluorescence levels per OD at different salt concentrations.

Figure.(C) Fluorescence images at different salt concentrations.

We set the salt concentration 0mm, 50mm, 100mm, 300mm, 500mm (NaCl). In Fig. 1, at the same OD value, the measured GFP fluorescence value increased with increasing salt concentration. In addition, green fluorescence at different salt concentrations can be visually observed, and it is concluded that an increase in salt concentration before 300mM NaCl enhances the expression of a salt-controlled promoter, an increase in the expression of green fluorescent protein, and an increase in GFP fluorescence value. At a salt concentration of 300 mM NaCl to 500 mM NaCl, the fluorescence intensity at the unit OD value is weakened. We speculate that higher salt concentrations have an adverse effect on cell growth and salt-controlled promoter expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 946