Difference between revisions of "Plasmid backbones"
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===[[Plasmids/Operating|Plasmids for use while operating or running BioBrick devices and systems]]=== | ===[[Plasmids/Operating|Plasmids for use while operating or running BioBrick devices and systems]]=== | ||
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Oftentimes, it pays to use a different set of plasmids for operating or running your BioBrick device or system than you use for assembly. For example, while high copy plasmids are great for getting good DNA yields when doing plasmid purification, some BioBrick devices and systems consume too many resources when operated on a high copy plasmid. In those cases, you might want to switch to a low or medium copy plasmid. | Oftentimes, it pays to use a different set of plasmids for operating or running your BioBrick device or system than you use for assembly. For example, while high copy plasmids are great for getting good DNA yields when doing plasmid purification, some BioBrick devices and systems consume too many resources when operated on a high copy plasmid. In those cases, you might want to switch to a low or medium copy plasmid. | ||
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===[[Plasmids/Construction|Parts for use in constructing new BioBrick vectors]]=== | ===[[Plasmids/Construction|Parts for use in constructing new BioBrick vectors]]=== | ||
| − | + | {| | |
| + | |{{Click || image=Toolman.png | link=Plasmids/Measurement |width=75px | height=120px }} | ||
| + | |Sometimes there just isn't a plasmid already available that has the replication origin and antibiotic resistance marker that you want. In that case, you might need to build a new BioBrick vector. But you don't need to start from scratch. Here are a set of BioBrick parts that might help you in building a new BioBrick vector. | ||
| + | |} | ||
===[[Plasmids/Other|Other plasmids that do not meet the BioBrick assembly standard but are still useful]]=== | ===[[Plasmids/Other|Other plasmids that do not meet the BioBrick assembly standard but are still useful]]=== | ||
| − | + | {| | |
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| + | |Some Registry contributors have built BioBrick plasmids that don't strictly comply with the BioBrick assembly standard, but still might be useful to you. Check out those plasmids here. | ||
| + | |} | ||
===Also see=== | ===Also see=== | ||
| − | [[Plasmids/Archive|Older plasmids that have been replaced by | + | [[Plasmids/Archive|Older plasmids that have been replaced by new versions of BioBrick plasmids]] |
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__NOEDITSECTION__ | __NOEDITSECTION__ | ||
Revision as of 21:00, 24 July 2008
Welcome to the one-stop shop for all plasmids and plasmid related parts.
(This page is a work in progress. Thank your for your patience. If you'd like to contribute, contact [http://openwetware.org/wiki/User:Reshma_P._Shetty Reshma Shetty])
Help!
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Plasmids for use while assembling BioBrick parts
| Are you trying to assemble two BioBrick parts together? There are a set of plasmids available designed to make assembly of BioBrick parts easier. |
Plasmids for use while measuring or characterizing BioBrick parts
| Are you interested in measuring the behavior of a BioBrick promoter, ribosome binding site, or transcriptional inverter? Jason Kelly has designed various plasmids to make the process of part characterization much easier. |
Plasmids for use while operating or running BioBrick devices and systems
Oftentimes, it pays to use a different set of plasmids for operating or running your BioBrick device or system than you use for assembly. For example, while high copy plasmids are great for getting good DNA yields when doing plasmid purification, some BioBrick devices and systems consume too many resources when operated on a high copy plasmid. In those cases, you might want to switch to a low or medium copy plasmid.
Parts for use in constructing new BioBrick vectors
| Sometimes there just isn't a plasmid already available that has the replication origin and antibiotic resistance marker that you want. In that case, you might need to build a new BioBrick vector. But you don't need to start from scratch. Here are a set of BioBrick parts that might help you in building a new BioBrick vector. |
Other plasmids that do not meet the BioBrick assembly standard but are still useful
| Some Registry contributors have built BioBrick plasmids that don't strictly comply with the BioBrick assembly standard, but still might be useful to you. Check out those plasmids here. |
Also see
Older plasmids that have been replaced by new versions of BioBrick plasmids