Difference between revisions of "Part:BBa K2587011"

Revision as of 17:24, 17 October 2018

P_RBS_gfp_T

This construct contains a constitutive promoter (J23102: https://parts.igem.org/Part:BBa_J23102), an RBS (B0032: https://parts.igem.org/Part:BBa_B0032), a fluorescent protein: GFP (E0040m: https://parts.igem.org/Part:BBa_E0040) and a terminator (B0015: https://parts.igem.org/Part:BBa_B0015). It is built using the CIDAR MoClo toolbox for Golden Gate cloning for E.coli and can be used to track the cells due to constitutive expression of a reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 706

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2587011 short</partinfo>
-This construct contains a constitutive promoter (J23102: https://parts.igem.org/Part:BBa_J23102),an RBS (B0032: https://parts.igem.org/Part:BBa_B0032), a fluorescent protein: GFP (E0040m: https://parts.igem.org/Part:BBa_E0040) and a terminator (B0015: https://parts.igem.org/Part:BBa_B0015).
+This construct contains a constitutive promoter (J23102: https://parts.igem.org/Part:BBa_J23102), an RBS (B0032: https://parts.igem.org/Part:BBa_B0032), a fluorescent protein: GFP (E0040m: https://parts.igem.org/Part:BBa_E0040) and a terminator (B0015: https://parts.igem.org/Part:BBa_B0015).
 It is built using the CIDAR MoClo toolbox for Golden Gate cloning for <i>E.coli</i> and can be used to track the cells due to constitutive expression of a reporter.