Difference between revisions of "Part:BBa K2621022"

 
 
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<partinfo>BBa_K2621022 short</partinfo>
 
<partinfo>BBa_K2621022 short</partinfo>
  
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[[Image:T--Vilnius-Lithuania-OG--catseqsmalllogo.jpeg|right|200px|<b>Figure 1. </b> Abstract scheme of the Catalytic Activity Sequencing]]
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CAT-Seq barcode collection consists of small DNA fragments with unique sequences that are used together with Nanopore Sequencing in Catalytic Activity Sequencing Pipeline. For example, if one wants to sequence two different libraries using the same Nanopore chip, it would be advised to barcode the libraries beforehand using the CAT-Seq barcodes. That is because DNA fragments from one sequencing run may present in the following one. If libraries are barcoded, these errors can be bioinformatically removed.
 +
 
 +
These barcodes can also be used in combination. For example, if a single barcode is used for each sample and there are 18 different barcodes to choose from, one can barcode 18 different samples. Yet, if used in combinations of two - up to 324 samples can potentially be barcoded using the same 18 original barcodes.
 +
 
 +
 
 +
Of course, these barcodes are multi-purpose and can be used for any other required application!
 +
 
 +
=CAT-Seq Barcode Inventory=
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 +
Here is the list of unique CAT-Seq barcodes and their sequences:
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 +
<center>
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{| class="wikitable"
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! style="text-align: center; font-weight:bold;" | Biocrick number
 +
! style="text-align: center; font-weight:bold;" | Part name
 +
! style="text-align: center; font-weight:bold;" | Barcode Sequence
 +
|-
 +
| [https://parts.igem.org/Part:BBa_K2621017 BBa_K2621017]
 +
| CAT-Seq Barcode 1
 +
| AAGAAAGTTGTCGGTGTCTTTGTG
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|-
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| [https://parts.igem.org/Part:BBa_K2621018 BBa_K2621018]
 +
| CAT-Seq Barcode 2
 +
| TCGATTCCGTTTGTAGTCGTCTGT
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|-
 +
| [https://parts.igem.org/Part:BBa_K2621019 BBa_K2621019]
 +
| CAT-Seq Barcode 3
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| GAGTCTTGTGTCCCAGTTACCAGG
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|-
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| [https://parts.igem.org/Part:BBa_K2621020 BBa_K2621020]
 +
| CAT-Seq Barcode 4
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| TAGGGAAACACGATAGAATCCGAA
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|-
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| [https://parts.igem.org/Part:BBa_K2621021 BBa_K2621021]
 +
| CAT-Seq Barcode 5
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| CTTGTCCAGGGTTTGTGTAACCT
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|-
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| [https://parts.igem.org/Part:BBa_K2621022 BBa_K2621022]
 +
| CAT-Seq Barcode 6
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| GACTACTTTCTGCCTTTGCGAGAA
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|-
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| [https://parts.igem.org/Part:BBa_K2621023 BBa_K2621023]
 +
| CAT-Seq Barcode 7
 +
| GTGTTACCGTGGGAATGAATCCTT
 +
|-
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| [https://parts.igem.org/Part:BBa_K2621024 BBa_K2621024]
 +
| CAT-Seq Barcode 8
 +
| TTCAGGGAACAAACCAAGTTACGT
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|-
 +
| [https://parts.igem.org/Part:BBa_K2621025 BBa_K2621025]
 +
| CAT-Seq Barcode 9
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| AACTAGGCACAGCGAGTCTTGGTT
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|-
 +
| [https://parts.igem.org/Part:BBa_K2621026 BBa_K2621026]
 +
| CAT-Seq Barcode 10
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| AAGCGTTGAAACCTTTGTCCTCTC
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|-
 +
| [https://parts.igem.org/Part:BBa_K2621027 BBa_K2621027]
 +
| CAT-Seq Barcode 11
 +
| GTTTCATCTATCGGAGGGAATGGA
 +
|-
 +
| [https://parts.igem.org/Part:BBa_K2621028 BBa_K2621028]
 +
| CAT-Seq Barcode 12
 +
| CAGGTAGAAAGAAGCAGAATCGGA
 +
|-
 +
| [https://parts.igem.org/Part:BBa_K2621029 BBa_K2621029]
 +
| CAT-Seq Barcode 13
 +
| TCAGTGAGGATCTACTTCGACCCA
 +
|-
 +
| [https://parts.igem.org/Part:BBa_K2621030 BBa_K2621030]
 +
| CAT-Seq Barcode 14
 +
| TGCGTACAGCAATCAGTTACATTG
 +
|-
 +
| [https://parts.igem.org/Part:BBa_K2621031 BBa_K2621031]
 +
| CAT-Seq Barcode 15
 +
| CCAGTAGAAGTCCGACAACGTCAT
 +
|-
 +
| [https://parts.igem.org/Part:BBa_K2621032 BBa_K2621032]
 +
| CAT-Seq Barcode 16
 +
| CAGACTTGGTACGGTTGGGTAACT
 +
|-
 +
| [https://parts.igem.org/Part:BBa_K2621033 BBa_K2621033]
 +
| CAT-Seq Barcode 17
 +
| GGACGAAGAACTCAAGTCAAAGGC
 +
|-
 +
| [https://parts.igem.org/Part:BBa_K2621034 BBa_K2621034]
 +
| CAT-Seq Barcode 18
 +
| CTACTTACGAAGCTGAGGGACTGC
 +
|}
 +
</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:40, 17 October 2018


CAT-Seq Barcode 6

Figure 1. Abstract scheme of the Catalytic Activity Sequencing

CAT-Seq barcode collection consists of small DNA fragments with unique sequences that are used together with Nanopore Sequencing in Catalytic Activity Sequencing Pipeline. For example, if one wants to sequence two different libraries using the same Nanopore chip, it would be advised to barcode the libraries beforehand using the CAT-Seq barcodes. That is because DNA fragments from one sequencing run may present in the following one. If libraries are barcoded, these errors can be bioinformatically removed.

These barcodes can also be used in combination. For example, if a single barcode is used for each sample and there are 18 different barcodes to choose from, one can barcode 18 different samples. Yet, if used in combinations of two - up to 324 samples can potentially be barcoded using the same 18 original barcodes.


Of course, these barcodes are multi-purpose and can be used for any other required application!

CAT-Seq Barcode Inventory

Here is the list of unique CAT-Seq barcodes and their sequences:

Biocrick number Part name Barcode Sequence
BBa_K2621017 CAT-Seq Barcode 1 AAGAAAGTTGTCGGTGTCTTTGTG
BBa_K2621018 CAT-Seq Barcode 2 TCGATTCCGTTTGTAGTCGTCTGT
BBa_K2621019 CAT-Seq Barcode 3 GAGTCTTGTGTCCCAGTTACCAGG
BBa_K2621020 CAT-Seq Barcode 4 TAGGGAAACACGATAGAATCCGAA
BBa_K2621021 CAT-Seq Barcode 5 CTTGTCCAGGGTTTGTGTAACCT
BBa_K2621022 CAT-Seq Barcode 6 GACTACTTTCTGCCTTTGCGAGAA
BBa_K2621023 CAT-Seq Barcode 7 GTGTTACCGTGGGAATGAATCCTT
BBa_K2621024 CAT-Seq Barcode 8 TTCAGGGAACAAACCAAGTTACGT
BBa_K2621025 CAT-Seq Barcode 9 AACTAGGCACAGCGAGTCTTGGTT
BBa_K2621026 CAT-Seq Barcode 10 AAGCGTTGAAACCTTTGTCCTCTC
BBa_K2621027 CAT-Seq Barcode 11 GTTTCATCTATCGGAGGGAATGGA
BBa_K2621028 CAT-Seq Barcode 12 CAGGTAGAAAGAAGCAGAATCGGA
BBa_K2621029 CAT-Seq Barcode 13 TCAGTGAGGATCTACTTCGACCCA
BBa_K2621030 CAT-Seq Barcode 14 TGCGTACAGCAATCAGTTACATTG
BBa_K2621031 CAT-Seq Barcode 15 CCAGTAGAAGTCCGACAACGTCAT
BBa_K2621032 CAT-Seq Barcode 16 CAGACTTGGTACGGTTGGGTAACT
BBa_K2621033 CAT-Seq Barcode 17 GGACGAAGAACTCAAGTCAAAGGC
BBa_K2621034 CAT-Seq Barcode 18 CTACTTACGAAGCTGAGGGACTGC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]