Difference between revisions of "Part:BBa K2718010"

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=== Design notes===
 
=== Design notes===
  
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It's a composite part whith [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] like RBS.
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It's a part whith [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] like RBS.
 
And coding sequence comes from ''Amycolatopsis orientalis''. And the sequence is optimize for E.coli thanks to  
 
And coding sequence comes from ''Amycolatopsis orientalis''. And the sequence is optimize for E.coli thanks to  
 
[https://eu.idtdna.com/CodonOpt IDT tool]
 
[https://eu.idtdna.com/CodonOpt IDT tool]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2718010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2718010 SequenceAndFeatures</partinfo>
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===Sources===
 
===Sources===
  

Revision as of 20:12, 16 October 2018


RBS (strong) Hma s

HmaS is a 4-hydroxymandelate synthase which catalise phenylpyruvate + O2 => (S)Mandelate + CO2

Activity test

See BBa_K2718011

Design notes

It's a part whith BBa_B0030 like RBS. And coding sequence comes from Amycolatopsis orientalis. And the sequence is optimize for E.coli thanks to IDT tool

This biobrick exists with IPTG inducible promotor BBa_K2718011

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 546
    Illegal BamHI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 495

Sources

Pugh, S., McKenna, R., Halloum, I., and Nielsen, D. R. (2015). EngineeringEscherichia coli for renewable benzyl alcohol production. Met. Eng. Commun.2, 39–45. doi: 10.1016/j.meteno.2015.06.002