Difference between revisions of "Part:BBa K2718010"

 
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<partinfo>BBa_K2718010 short</partinfo>
 
<partinfo>BBa_K2718010 short</partinfo>
  
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A
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HmaS is a 4-hydroxymandelate synthase wich catalize phenylpyruvate + O2  => (S)Mandelate + CO2
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===Activity test===
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See [https://parts.igem.org/Part:BBa_K2718010 BBa_K2718010]
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=== Design notes===
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It's a composite part whith [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] like RBS.
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And coding sequence comes from ''Amycolatopsis orientalis''. And the sequence is optimize for E.coli thanks to
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[https://eu.idtdna.com/CodonOpt IDT tool]
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This biobrick exists with IPTG inducible promotor [https://parts.igem.org/Part:BBa_K2718011 BBa_K2718011]
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2718010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2718010 SequenceAndFeatures</partinfo>
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===Sources===
  
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Pugh, S., McKenna, R., Halloum, I., and Nielsen, D. R. (2015). EngineeringEscherichia coli for renewable benzyl alcohol production. Met. Eng. Commun.2, 39–45. doi: 10.1016/j.meteno.2015.06.002
  
 
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Revision as of 14:33, 15 October 2018


RBS (strong) Hma s

HmaS is a 4-hydroxymandelate synthase wich catalize phenylpyruvate + O2 => (S)Mandelate + CO2

Activity test

See BBa_K2718010

Design notes

It's a composite part whith BBa_B0030 like RBS. And coding sequence comes from Amycolatopsis orientalis. And the sequence is optimize for E.coli thanks to IDT tool

This biobrick exists with IPTG inducible promotor BBa_K2718011

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 546
    Illegal BamHI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 495

Sources

Pugh, S., McKenna, R., Halloum, I., and Nielsen, D. R. (2015). EngineeringEscherichia coli for renewable benzyl alcohol production. Met. Eng. Commun.2, 39–45. doi: 10.1016/j.meteno.2015.06.002