Difference between revisions of "Part:BBa K2660008"
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| − | This part was engineered to be a building block of the standard BioBrick framework, and can be used with pMag (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2660009">BBa_K2660009</a></html>) to join different domains of a protein of interest when irradiated by blue light | + | This part was engineered to be a building block of the standard BioBrick framework, and can be used with pMag (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2660009">BBa_K2660009</a></html>) to join different domains of a protein of interest when irradiated by blue light like N-cas9 (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2660006">BBa_K2660006</a></html>) and C-cas9 (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2660007">BBa_K2660007</a></html>) domains |
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Revision as of 13:58, 14 October 2018
nMag
- Light-activated protein
This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools. The negative Magnet (nMag) when irradiated by a blue light change through electrostatic interactions.
This part was engineered to be a building block of the standard BioBrick framework, and can be used with pMag (BBa_K2660009) to join different domains of a protein of interest when irradiated by blue light like N-cas9 (BBa_K2660006) and C-cas9 (BBa_K2660007) domains
References
Kawano, F. et al. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat. Commun. 6:6256 doi: 10.1038/ncomms7256 (2015)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 87
- 1000COMPATIBLE WITH RFC[1000]